Harry Grimoire is a normal kid until Felicia finds him in the first episode and tells him about his ancestor's legacy and that he is the current descendant of the Grimoire family, meaning that he has magic powers. He starts to learn how to use his powers and in Darkest Before The Dawn he is finally given his full powers by the spirits of his descendants. Harry was made just for the cartoon and is not in the games.

Felicia is the main good Darkstalker in the series and always follows/leads Harry around in each episode. She's a catwoman and was in the showbiz game until she was tracked down by Demitri who wanted her to join Pyron's side. She refuses and eventually finds a descendant of a great sorcerer called Harry in the first episode.
Cartoon Felicia has more fur to make her less revealing, but other than that she looks a lot like her game counterpart. Whenever she gets badly hurt she'll morph into a smaller cat form, although she can also do this at will as shown in the first episode.

Rikou is the last of the fishmen race, and lives under the sea in Atlantis. However, when Pyron arrives he is found by Demitri, who wants him to join Pyron's side. Rikou refuses and joins up with the good Darkstalkers.
In some episodes, Rikou seems to require to be in water to survive, but in other episodes this isn't a problem. A running gag is that other characters will call him "strangely attractive for a fishman" with the likes of Donovan and Terramon saying it, strangely.
In the Japanese versions of the games, he's known as Aulbath.

APPEARANCES: Out of the Dark, Pyramid Power, The Game, Ghost Hunter, Little Bigfoot's Last Stand, Aliens Keep Out, My Harry's in the Highlands, Samurai's Honor, There's No Business Like Dragon Business, Darkest Before The Dawn, Everyone's A Critic

Bigfoot is the leader of a Sasquatch tribe. When Demitri finds the tribe, Bigfoot gets his people to throw snowballs at the beam which disables it. Later on he allies with Rikou in an attempt to start a resistance to Pyron, and eventually joins up with the other good Darkstalkers.
For reasons unknown he's had a name change, he's called Sasquatch in the games. Also, his game version is a lot more fierce looking with red eyes, although he's actually quite friendly. The cartoon version is still perfectly nice, although they gave him human eyes. In the later episodes, he (and everyone in his tribe) have black eyes. Bigfoot also has a nephew called Hairball.

APPEARANCES: Out of the Dark, The Game, Little Bigfoot's Last Stand, There's No Business Like Dragon Business, Everyone's A Critic

Bishamon is a samurai who was cursed long a go thanks to an evil shogun he was under the command of. Locked in a bell for centuries, he was finally freed when Demitri used the beam to send him up to Pyron's spaceship. Bishamon was still cursed however and could not see the spirit of his dead wife, Orin. Despite being on the bad side he still had a strong sense of honour and prevented Morrigan from attacking Rikou in Aliens Keep Out. After the events of Samurai's Honor he joins the good side.
In the games, his story is quite a bit more sinister. The armour he wears controls Bishamon and he must kill at least one person a day to prevent the armour from taking his own life. Of course, something like that would never make it into a children's cartoon.

APPEARANCES: Out of the Dark, The Game, Ghost Hunter, Little Bigfoot's Last Stand, Aliens Keep Out, Samurai's Honor, Darkest Before The Dawn, Everyone's A Critic

Donovan Baine is referred to as the "Dark Hunter" and hunts down the Darkstalkers, not caring if they are good or bad. No backstory is revealed for him throughout the series but, as in the games, he uses a sword in fights. Also like in the games, he has a little girl follow him around (Amanda) in the later episodes. The cartoons spell his name as Donavon, reversing the last two vowels.
Donovan's story is much, much more complicated in the games. In the games, he's half human, half vampire, whilst in the cartoon he's completely human. His game counterpart struggles to battle his own dark side but this is never mentioned in the cartoon. In the games one of his eyes glows yellow when he uses his power, but again this isn't in the cartoon.

APPEARANCES: Donovan's Bane, Aliens Keep Out, Samurai's Honor, Darkest Before The Dawn, Everyone's A Critic

Amanda is a little girl who has her parents killed by Demitri in Samurai's Honor. She is nearly killed as well, but thanks to Donovan she is saved. However, she is blasted by Donovan's sword which gives her the power to seek out the Darkstalkers, so Donovan is forced to take her with him on his quest to destroy the Darkstalkers.
In the games, she is known as Anita. She has brown hair opposed to the blonde hair she has in the cartoon. The game version of Anita carries a headless doll around with her at all times, but in the cartoon she is only ever shown with a small cuddly black dinosaur. Her backstory in the games is also completely different. Amanda never speaks a word in the series.

APPEARANCES: Samurai's Honor, Darkest Before The Dawn

Victor von Gerdenheim is awakened when he is nearly beamed up to Pyron's spaceship, but he escapes. Finding out that he has been used as a movie prop for years, he returns home to his castle and helps out the good Darkstalkers when he can.
Victor's main appearance is in The Game, he then isn't in any episodes until the last one. His back story is pretty different to the games as in those he was a creation of a scientist, whilst in the cartoons, he seems to be the latest descendant in a family. It's also worth noting that the animator's gave him his Night Warriors default costume (red jacket) opposed to his Darkstalker's default costume (green jacket). Nearly all the other characters colours were based on their appearance in the first Darkstalkers game.

APPEARANCES: Out of the Dark, The Game, Everyone's A Critic

Jon Talbain is a werewolf, and that is all I can really say on him as very little is ever revealed about him in the series. His main episode is My Harry's in the Highlands where he has a flashback of a town exploding, but it's never touched upon again. All that can be said is that he fights on the side of good.
As in the games, Talbain can change into a human, although this doesn't quite make sense in the cartoon as shown in the above noted episode. In the Japanese versions of the games, he is called Gallon.

APPEARANCES: Out of the Dark, The Game, My Harry's in the Highlands, Everyone's A Critic

Hsien-Ko is another hunter who is on a quest to destroy Demitri, who killed her parents and her sister Mei-Ling. She appears in two episodes and as in the games she attacks with many different weapons, although they often look stupid in the cartoon.
Hsien-Ko herself has normal skin opposed to the blue skin she has in the games. She also has rather ridiculous "Dr. Claw" hands which are quite different to the claws she uses in the games. In the Japanese versions of the games, Hsien-Ko is called Lei Lei. Mei-Ling is known as Lin Lin in the games, and in the games she survives to fight with her sister.

APPEARANCES: Ghost Hunter, Darkest Before The Dawn, Everyone's A Critic

Huitzil is the name of a robot built to defend the Earth against aliens. When Pyron beams himself down to Earth in Aliens Keep Out, Huitzil is activated automatically and tries to sacrifice Pyron to the sun god. Thanks to the combined efforts of Morrigan, Rikou and Bishamon he is stopped and blown apart, although he starts to pull himself back together at the end of the episode.
Game Huitzil has a completely different story - it's the generic name for an army of robots which Pyron himself built to destroy the dinosaurs on Earth millions of years a go. The one featured in the cartoon is made to kill Pyron though. Cartoon Huitzil is also coloured blue like he is in Night Warriors, although he was bronze in the first Darkstalkers game. In the Japanese versions of the games, he's known as Phobos.

APPEARANCES: Aliens Keep Out

Herv is Raptor's luckless agent. He is often abused by Raptor but keeps trying to get him gigs, although the gigs are often complete disasters. However, he eventually manages to get Raptor into the movie business.

APPEARANCES: And The Walls Come Tumblin' Down, There's No Business Like Dragon Business

Hairball is Bigfoot's nephew and his only purpose in the show is to cause trouble. Wanting to know more about the world he usually asks hundreds of questions to whoever he meets and gets on their nerves, and wants to see some action like the rest of the good Darkstalkers do.

APPEARANCES: Out of the Dark, Little Bigfoot's Last Stand, There's No Business Like Dragon Business

Klaus is Victor's servant. His family have served the von Gerdenheims for generations. He appears in two episodes, but his main appearance is in The Game. He is not featured in the games and was made for this series.

APPEARANCES: Out of the Dark, The Game

Demitri Maximoff is the lord of the vampires and is awakened by Pyron. He is given the rank of general but this isn't really mentioned much at all in the later episodes. Anyway, Demitri is the main bad guy of the show, simply because he gets up to more no good than Pyron ever does and has far more fights than him.
For whatever reason, they decided to give him grey skin in the cartoon, this isn't the case with his game sprite. Cartoon Demitri also has much taller, orange hair. In the early episode of the cartoon his eyes have pupils, but these were removed later on.

APPEARANCES: Out of the Dark, Pyramid Power, The Game, And The Walls Come Tumblin' Down, Ghost Hunter, Little Bigfoot's Last Stand, Samurai's Honor, There's No Business Like Dragon Business, Darkest Before The Dawn, Everyone's A Critic

Morrigan Aensland is a succubus. In the cartoon her back story reveals that 200 years before Pyron's spaceship arrives she was sealed in a rock by a couple of white witches, and then the rock was tossed into an ocean. When Pyron and Demitri find her and beam her up, she instantly recognises Demitri and fights him. This sort of rivalry continues throughout the series.
Her cartoon version has been made a lot less attractive and her costume has been altered to be less revealing. Her game storyline is also completely ignored in the cartoon (since in the games, she isn't evil) and like Demitri, she is more of a threat than Pyron as she gets up to more no good than he does, including summoning her ancestor Morgan Le Fey and resurrecting Scotland's mightiest warriors.

APPEARANCES: Out of the Dark, Donovan's Bane, Pyramid Power, The Game, And The Walls Come Tumblin' Down, Ghost Hunter, Little Bigfoot's Last Stand, Aliens Keep Out, My Harry's in the Highlands, There's No Business Like Dragon Business, Darkest Before The Dawn, Everyone's A Critic

Pyron returns to Earth after last being there four thousand years a go. He is the self proclaimed "intergalactic collector of planets" (even though he only ever seems interested in getting the Earth) and when he returns to it he summons the Darkstalkers to do his bidding.
It's revealed in Aliens Keep Out that Pyron is a lot less powerful whenever he leaves his spaceship, so that explains why almost all of the time he's stuck on his own spaceship and never gets to do any fighting, usually sending Demitri or the other Darkstalkers on his side to do his bidding instead.

APPEARANCES: Out of the Dark, Pyramid Power, The Game, And The Walls Come Tumblin' Down, Ghost Hunter, Little Bigfoot's Last Stand, Aliens Keep Out, Samurai's Honor, There's No Business Like Dragon Business, Darkest Before The Dawn, Everyone's A Critic

Anakaris was one of Egypt's mightiest pharaohs, and when he died he was wrapped up in bandages and buried. When Pyron arrives thousands of years later, Anakaris is bought back to life as a Mummy to serve as one of his troops.
Anakaris is basically the comic relief for the evil Darkstalkers, as unlike his game counterpart he's a complete moron in this series, constantly getting himself confused or doing idiotic things like talking to penguins. However he does seem to be pretty powerful, having been given the ability to travel through time (see Pyramid Power and Everyone's A Critic), a skill he never had in the games.

APPEARANCES: Out of the Dark, Pyramid Power, The Game, And The Walls Come Tumblin' Down, Little Bigfoot's Last Stand, Everyone's A Critic

Raptor is the name of an undead rock guitarist who died (in the cartoon) in a plane crash, but when Pyron returns he is resurrected as zombie and joins Pyron's side.
However eventually Raptor starts to do things his own way and gets a agent called Herv to try and make him famous, but all the gigs he get are always awful with no crowds, and it doesn't help that nobody likes Raptor's music. Raptor also makes various power plays to try and make himself the star throughout the series, including trying to take the trumpet of Jericho and getting a dragon to help him out. In the Japanese games, Raptor is called Zabel.

APPEARANCES: Out of the Dark, The Game, And The Walls Come Tumblin' Down, Ghost Hunter, Little Bigfoot's Last Stand, There's No Business Like Dragon Business, Everyone's A Critic

Ship is the name of the small sphere object that controls Pyron's spaceship. It has a mind of it's own and often takes digs at Pyron for his blunders. However, it does actually care for Pyron in episodes such as "Alien's keep out" where it gathers a few Darkstalkers to save him from Huitzil.

APPEARANCES: Out of the Dark, Pyramid Power, The Game, And The Walls Come Tumblin' Down, Ghost Hunter, Little Bigfoot's Last Stand, Aliens Keep Out, Samurai's Honor, There's No Business Like Dragon Business, Darkest Before The Dawn, Everyone's A Critic Everyone's A Critic

Terramon is Pyron's older brother, and is the head of the family and much stronger than Pyron. He goes to check on his little brother's progress in the final episode of the series, but after seeing how humans could become a good food source, he wants to take it over for himself, but is thwarted by the combined efforts of the good and bad Darkstalkers. He was made just for this series and is not in the games.

APPEARANCES: Everyone's A Critic